4‐Hydroxyderricin, as a PPARγ Agonist, Promotes Adipogenesis, Adiponectin Secretion, and Glucose Uptake in 3T3‐L1 Cells

• DOI: 10.1007/s11745-016-4154-9
• Publication Date: 2016-04-20T05:54:49Z
• Authors: Yongjia Li; Tsuyoshi Goto; Kanae Yamakuni; Haruya Takahashi; Nobuyuki Takahashi; Huei‐Fen Jheng; Wataru Nomura; Masahiko Taniguchi; Kimiye Baba; Shigeru Murakami; Teruo Kawada
• Keywords: 0303 health sciences; Adipogenesis; Glucose Transporter Type 4; Plant Extracts; Cell Differentiation; PPAR gamma; Mice; 03 medical and health sciences; Chalcone; Glucose; Gene Expression Regulation; 3T3-L1 Cells; Adipocytes; Animals; Adiponectin; Phosphorylation; Proto-Oncogene Proteins c-akt; Angelica

AbstractAdipocyte differentiation plays a pivotal role in maintaining the production of small‐size adipocytes with insulin sensitivity, and impaired adipogenesis is implicated in insulin resistance. 4‐Hydroxyderricin (4‐HD), a phytochemical component of Angelica keiskei, possesses diverse biological properties such as anti‐inflammatory, antidiabetic, and antitumor. In the present study, we investigated the effects of 4‐HD on adipocyte differentiation. 4‐HD promoted lipid accumulation in 3T3‐L1 cells, upregulated both peroxisome proliferator‐activated receptor (PPAR)‐γ mRNA and protein expression, and acted as a ligand for PPARγ in the luciferase assay. Moreover, 4‐HD increased the mRNA and protein expression levels of adiponectin. Additionally, it promoted insulin‐dependent glucose uptake into 3T3‐L1 adipocytes and increased Akt phosphorylation and glucose transporter (GLUT) 4 mRNA expression. In summary, these findings suggest that 4‐HD, which promoted adipogenesis and insulin sensitivity in 3T3‐L1 cells, might be a phytochemical with potent insulin‐sensitizing effects.