Measurement of Ether Phospholipids in Human Plasma with HPLC–ELSD and LC/ESI–MS After Hydrolysis of Plasma with Phospholipase A1

• DOI: 10.1007/s11745-016-4170-9
• Publication Date: 2016-07-07T06:18:46Z
• Authors: Shiro Mawatari; Seira Hazeyama; Takehiko Fujino
• Keywords: Phospholipase A1; Chromatography detector

Ethanolamine ether phospholipid (eEtnGpl) and choline (eChoGpl) are present in human plasma or serum, but the relative concentration of phospholipids is very low as compared to those other tissues. Nowadays, measurement depends on tandem mass spectrometry (LC/MS/MS), a system for LC/MS/MS generally too expensive usual clinical laboratories. Treatment with phospholipase A1 (PLA1) causes complete hydrolysis diacylphospholipids, remain intact. After treatment PLA1, both eEtnGpl eChoGpl detected independent peaks by high-performance liquid chromatography evaporative light scattering detection (HPLC-ELSD). The same sample used HPLC-ELSD can be applied detect electrospray ionization spectrometry. Presence alkylacylphospholipids was indicated sequential PLA1 hydrochloric acid.