Female Mice are Resistant to Fabp1 Gene Ablation‐Induced Alterations in Brain Endocannabinoid Levels

• DOI: 10.1007/s11745-016-4175-4
• Publication Date: 2016-07-23T07:22:31Z
• Authors: Gregory G. Martin; Sarah Chung; Danilo Landrock; Kerstin K. Landrock; Lawrence J. Dangott; Xiaoxue Peng; Martin Kaczocha; Eric J. Murphy; Ann B. Kier; Friedhelm Schroeder
• Keywords: Male; 0301 basic medicine; Mice; 0303 health sciences; 03 medical and health sciences; Arachidonic Acid; Ethanolamines; Animals; Brain; Female; Fatty Acid-Binding Proteins; Endocannabinoids; Glycerides

Although liver fatty acid binding protein (FABP1, L-FABP) is not detectable in the brain, Fabp1 gene ablation (LKO) markedly increases endocannabinoids (EC) brains of male mice. Since brain EC system females differs significantly from that males, it was important to determine if LKO differently impacted system. did alter levels arachidonic (ARA)-containing EC, i.e. arachidonoylethanolamide (AEA) and 2-arachidonoylglycerol (2-AG), but decreased non-ARA-containing N-acylethanolamides (OEA, PEA) 2-oleoylglycerol (2-OG) potentiate actions AEA 2-AG. These changes potentiating were associated with: (1) a net decrease membrane proteins with uptake synthesis; (2) increase cytosolic chaperones enzymes degradation; or (3) increased receptors (CB1, TRVP1). Instead, reduced opposite responsiveness female loss FABP1 correlated intrinsically lower level livers WT than males. data show mouse endocannabinoid unchanged (AEA, 2-AG) PEA, 2-OG) by complete (LKO).