Characterization of Cross‐Linked Lipase Aggregates

• DOI: 10.1007/s11746-009-1401-8
• Publication Date: 2009-05-26T17:46:52Z
• Authors: B. L. A. Prabhava...; Zheng Guo; Xuebing Xu
• Keywords: Candida antarctica; Glutaraldehyde; Ammonium sulfate

Abstract Commercially available microbial lipases from different sources were immobilized as cross‐linked enzyme aggregates (CLEAs) using precipitants and glutaraldehyde cross‐linkers. These CLEAs assayed based on esterification between lauric acid n ‐propanol in solvent‐free systems. Precipitants found to have a profound influence both specific activities total activity recovery of CLEAs, exemplified by Candida antarctica lipase B (CALB). Among the CALB studied, those obtained PEG600, ammonium sulfate, PEG200 acetone observed attain over 200% comparison with powder directly precipitated liquid solution acetone. CLEA gave best (139% relative powder). The results kinetic studies showed that V max / K m does not significantly change upon formation. This work presents characterization an assay, which is useful for exploring synthetic application potential technology favorable perspectives.