Lipase Induction in Yarrowia lipolytica for Castor Oil Hydrolysis and Its Effect on γ‐Decalactone Production

• DOI: 10.1007/s11746-011-1987-5
• Publication Date: 2011-12-27T07:45:10Z
• Authors: Adelaide Braga; Nelma Gomes; Isabel Belo
• Keywords: Yarrowia; Ricinoleic acid; Biotransformation; Aroma compound; Inducer

Abstract γ‐Decalactone is an aromatic compound of industrial interest, resulting from the biotransformation ricinoleic acid, major constituent castor oil. In order to increase availability substrate cells for aroma production, oil previously hydrolyzed can be used. This hydrolysis may promoted by enzymatic action, more specifically lipases. this work, influence upon production lipase produced Yarrowia lipolytica , a microorganism able carry out biotransformation, was studied. first approach, induction conditions were analyzed using different Y . strains and culture conditions, such as inoculation mode medium. Lipase not affected centrifugation, so step eliminated, reducing time phases process. Moreover, W29 shown most adequate strain production. To investigate importance hydrolysis, pre‐addition inducer (olive oil) medium tested. Results showed that highest (1,600 mg L −1 ) obtained without inducer. However, pre‐induction decreased lag phase γ‐decalactone secretion.