Single Tryptophan of Disordered Loop from Plasmodium falciparum Purine Nucleoside Phosphorylase: Involvement in Catalysis and Microenvironment

• DOI: 10.1007/s12010-013-0228-9
• Publication Date: 2013-04-30T22:29:31Z
• Authors: Manish Kumar Suthar; Anita Verma; Pawan Kumar Doharey; Shiv Vardan Singh; Jitendra Kumar Sa...
• Keywords: 0301 basic medicine; 0303 health sciences; Molecular Sequence Data; Plasmodium falciparum; Static Electricity; Protozoan Proteins; Tryptophan; Anilino Naphthalenesulfonates; Catalysis; Protein Structure, Secondary; 3. Good health; Enzyme Activation; 03 medical and health sciences; Purine-Nucleoside Phosphorylase; Catalytic Domain; Amino Acid Sequence; Conserved Sequence; Bromosuccinimide; Protein Binding

Among various tropical diseases, malaria is a major life-threatening disease caused by Plasmodium parasite. Plasmodium falciparum is responsible for the deadliest form of malaria, so-called cerebral malaria. Purine nucleoside phosphorylase from P. falciparum is a homohexamer containing single tryptophan residue per subunit that accepts inosine and guanosine but not adenosine for its activity. This enzyme has been exploited as drug target against malaria disease. It is important to draw together significant knowledge about inherent properties of this enzyme which will be helpful in better understanding of this drug target. The enzyme shows disorder to order transition during catalysis. The single tryptophan residue residing in conserved region of transition loop is present in purine nucleoside phosphorylases throughout the Plasmodium genus. This active site loop motif is conserved among nucleoside phosphorylases from apicomplexan parasites. Modification of tryptophan residue by N-bromosuccinamide resulted in complete loss of activity showing its importance in catalysis. Inosine was not able to protect enzyme against N-bromosuccinamide modification. Extrinsic fluorescence studies revealed that tryptophan might not be involved in substrate binding. The tryptophan residue localised in electronegative environment showed collisional and static quenching in the presence of quenchers of different polarities.