Canine Parvovirus Type 2a (CPV-2a)-Induced Apoptosis in MDCK Involves Both Extrinsic and Intrinsic Pathways
0303 health sciences
Parvovirus, Canine
Cell Membrane
Apoptosis
Biological Transport
Phosphatidylserines
Endoplasmic Reticulum
Diploidy
Madin Darby Canine Kidney Cells
Nucleosomes
3. Good health
03 medical and health sciences
Dogs
Caspases
Animals
Tumor Suppressor Protein p53
Signal Transduction
DOI:
10.1007/s12010-013-0538-y
Publication Date:
2013-10-03T01:29:48Z
AUTHORS (19)
ABSTRACT
The canine parvovirus type 2 (CPV-2) causes an acute disease in dogs. It has been found to induce cell cycle arrest and DNA damage leading to cellular lysis. In this paper, we evaluated the apoptotic potential of the "new CPV-2a" in MDCK cells and elucidated the mechanism of the induction of apoptosis. The exposure of MDCK cells to the virus was found to trigger apoptotic response. Apoptosis was confirmed by phosphatidylserine translocation, DNA fragmentation assays, and cell cycle analysis. Activation of caspases-3, -8, -9, and -12 and decrease in mitochondrial potential in CPV-2a-infected MDCK cells suggested that the CPV-2a-induced apoptosis is caspase dependent involving extrinsic, intrinsic, and endoplasmic reticulum pathways. Increase in p53 and Bax/Bcl2 ratio was also observed in CPV-2a-infected cells.
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