Canine Parvovirus Type 2a (CPV-2a)-Induced Apoptosis in MDCK Involves Both Extrinsic and Intrinsic Pathways

• DOI: 10.1007/s12010-013-0538-y
• Publication Date: 2013-10-03T01:29:48Z
• Authors: Juwar Doley; Lakshya Veer Singh; G. Ravi Kumar; Aditya Prasad Sahoo; Lovleen Saxena; Uttara Chaturvedi; Shikha Saxena; Rajiv Kumar; Prafull Kumar Singh; R. S. Rajmani; Lakshman Santra; S. K. Palia; S. Tiwari; D. R. Harish; Arvind Kumar; G. S. Desai; Smita Gupta; Shishir K. Gupta; A. K. Tiwari
• Keywords: 0301 basic medicine; 0303 health sciences; Parvovirus, Canine; Cell Membrane; Apoptosis; Biological Transport; Phosphatidylserines; Endoplasmic Reticulum; Diploidy; Madin Darby Canine Kidney Cells; Nucleosomes; 3. Good health; 03 medical and health sciences; Dogs; Caspases; Animals; Tumor Suppressor Protein p53; Signal Transduction

The canine parvovirus type 2 (CPV-2) causes an acute disease in dogs. It has been found to induce cell cycle arrest and DNA damage leading to cellular lysis. In this paper, we evaluated the apoptotic potential of the "new CPV-2a" in MDCK cells and elucidated the mechanism of the induction of apoptosis. The exposure of MDCK cells to the virus was found to trigger apoptotic response. Apoptosis was confirmed by phosphatidylserine translocation, DNA fragmentation assays, and cell cycle analysis. Activation of caspases-3, -8, -9, and -12 and decrease in mitochondrial potential in CPV-2a-infected MDCK cells suggested that the CPV-2a-induced apoptosis is caspase dependent involving extrinsic, intrinsic, and endoplasmic reticulum pathways. Increase in p53 and Bax/Bcl2 ratio was also observed in CPV-2a-infected cells.