Production and Characterization of β-Glucanase Secreted by the Yeast Kluyveromyces marxianus

• DOI: 10.1007/s12010-013-0683-3
• Publication Date: 2014-01-03T07:03:34Z
• Authors: Mariana R. Lopes; Carlos J. A. de S...; Marina Q. R. B. R...; Daniela A. Costa; Ancély F. dos Santos; Leandro L. de Oli...; Humberto J. O. Ramos; Valéria M. Guimarães; Wendel B. Silveira; Flávia M. L. Passos; Luciano G. Fietto
• Keywords: 0106 biological sciences; beta-Glucans; Glycoside Hydrolases; Molecular Sequence Data; Temperature; Saccharomyces cerevisiae; Hydrogen-Ion Concentration; 01 natural sciences; Substrate Specificity; Fungal Proteins; Kinetics; Kluyveromyces; Glucose; Cell Wall; Fermentation; Amino Acid Sequence; Factor Analysis, Statistical; Sequence Alignment

An extracellular β-glucanase secreted by Kluyveromyces marxianus was identified for the first time. The optimal conditions for the production of this enzyme were evaluated by response surface methodology. The optimal conditions to produce β-glucanase were a glucose concentration of 4% (w/v), a pH of 5.5, and an incubation temperature of 35 °C. Response surface methodology was also used to determine the pH and temperature required for the optimal enzymatic activity. The highest enzyme activity was obtained at a pH of 5.5 and a temperature of 55 °C. Furthermore, the enzyme was partially purified and sequenced, and its specificity for different substrates was evaluated. The results suggest that the enzyme is an endo-β-1,3(4)-glucanase. After optimizing the conditions for β-glucanase production, the culture supernatant was found to be effective in digesting the cell wall of the yeast Saccharomyces cerevisiae, showing the great potential of β-glucanase in the biotechnological production of soluble β-glucan.