Impact of Selenium Deficiency on Inflammation, Oxidative Stress, and Phagocytosis in Mouse Macrophages

• DOI: 10.1007/s12011-019-01775-7
• Publication Date: 2019-06-19T22:02:18Z
• Authors: Jianwen Xu; Yafan Gong; Yue Sun; Jingzeng Cai; Qi Liu; Jun Bao; Jie Yang; Ziwei Zhang
• Keywords: Inflammation; 0301 basic medicine; Mice; Oxidative Stress; Selenium; 03 medical and health sciences; Phagocytosis; Macrophages; Animals; Cytokines; Cells, Cultured; 3. Good health

Although it has been reported that selenium (Se) deficiency can trigger inflammation, however, there are few reports on the effect of Se on the function of mouse peritoneal macrophages. Herein, we examined the expression of inflammatory factors, oxidative stress levels, and phagocytosis for primary-cultured peritoneal macrophages using control and Se-deficient groups. Our results revealed that Se deficiency induced the accumulation of oxygen free radicals and weakened antioxidant capacity. Se deficiency also significantly increased the expression of inflammation factors including iNOS, IL-1β, IL-12, IL-10, PTGe, and NF-κB. Meanwhile, Se suppression restrained macrophage production of TNF-α. The results of the phagocytosis assay demonstrated that Se deficiency inhibited the phagocytosis of macrophages. In conclusion, Se-deficient macrophages undergo severe inflammation through the NF-κB pathway due to the accumulation of oxygen free radicals and are hindered in their phagocytic capacity.