Novel Evidence that Purinergic Signaling - Nlrp3 Inflammasome Axis Regulates Circadian Rhythm of Hematopoietic Stem/Progenitor Cells Circulation in Peripheral Blood

• DOI: 10.1007/s12015-020-09953-0
• Publication Date: 2020-01-15T03:02:32Z
• Authors: Mateusz Adamiak; Andrzej Ciechanowicz; Marta Skoda; Monika Cymer; Michal Tracz; Bing Xu; Mariusz Z. Ratajczak
• Keywords: AIM2

Abstract We found that circadian changes in ATP level peripheral blood (PB) activate the Nlrp3 inflammasome, which triggers diurnal release of hematopoietic stem/progenitor cells (HSPCs) from murine bone marrow (BM) into PB. Consistent with this finding, we observed expression mRNA for inflammasome-related genes, including Nlrp3, caspase 1, IL-1β, IL-18, gasdermin (GSDMD), HMGB1, and S100A9. Circadian HSPCs BM PB as well Nlrp3-associated genes was decreased mice pannexin 1-mediated secretion inhibited by blocking peptide 10Panx animals exposed to specific small-molecule inhibitor inflammasome MCC950. In addition HSPCs, a similar decrease cell counts mesenchymal stromal (MSCs), endothelial progenitor (EPCs), very small embryonic-like stem (VSELs). These results shed more light on complexity regulation HSPC PB, is coordinated purinergic signaling-, innate immunity-dependent manner. Moreover, also other inflammasomes, Aim2, Nrp1a, Nlrp1b.