Comparison of the Variability of Small Extracellular Vesicles Derived from Human Liver Cancer Tissues and Cultured from the Tissue Explants Based on a Simple Enrichment Method

• DOI: 10.1007/s12015-021-10264-1
• Publication Date: 2021-09-22T11:04:10Z
• Authors: Jie Chen; Zhigang Jiao; Jianwen Mo; Defa Huang; Zhengzhe Li; Wenjuan Zhang; Tong Yang; Minghong Zhao; Fangfang Xie; Die Hu; Xiaoxing Wang; Xiaomei Yi; Yu Jiang; Tianyu Zhong
• Keywords: Extracellular Vesicles; Liver tissue

Abstract A potential use of small extracellular vesicles (sEVs) for diagnostic and therapeutic purposes has recently generated a great interest. sEVs, when purified directly from various tissues with proper procedures, can reflect the physiological pathological state organism. However, quality sEV is affected by many factors during isolation, including separation cell debris, enzymes tissue digestion, storage tissues. In present study, we established an assay isolation purification liver cancer tissues-derived sEVs (tdsEVs) cultured explants-derived (cedsEVs) comparing derived different concentration digestion enzyme incubation time. The nano-flow cytometry (NanoFCM) showed that isolated tdsEVs our method are purer than those obtained differential ultracentrifugation. Our study thus establishes simple effective approach high-quality be used analysis their constituents. Graphical abstract