hUC-MSCs Prevent Acute High-Altitude Injury through Apoe/Pdgf-b/p-Erk1/2 Axis in Mice

• DOI: 10.1007/s12015-024-10840-1
• Publication Date: 2025-01-28T03:06:40Z
• Authors: Siyu Yan; Youkun Bi; Qun Liu; Shaole Song; Lihong Ma; Guangju Ji

The hypobaric hypoxic atmosphere can cause adverse reactions or sickness. purpose of this study was to explore the preventive effect and mechanism human umbilical cord mesenchymal stem cells (hUC-MSCs) on acute pathological injury in mice exposed high-altitude. We pretreated C57BL/6 with hUC-MSCs via tail vein injection, then were subjected conditions for five days. effects lung, heart, brain assessed by biochemical analysis, histopathological testing, quantitative real-time polymerase chain reaction (qPCR), western blot (WB). Further, transcriptome sequencing used screen potential therapeutic targets injury, identified signaling axis characterized using Apoe−/− mice, qPCR WB. administration notably prevented relieved gastrointestinal symptoms inflammation lung increased blood oxygen saturation serum superoxide dismutase (SOD) level, decreased malondialdehyde (MDA) rescued tissue myocardial mitochondrial disorder, elevated nissl bodies number reduced degree pulmonary cerebral edema. Furthermore, pretreatment reversed down-regulated Apoe up-regulated Pdgf-b p-Erk1/2 mice. Thus, protected against caused condition Apoe/Pdgf-b/p-Erk1/2 axis, pathway confirmed negative results possess hypoxia environment at