Bone turnover is altered during 72 h of sleep restriction: a controlled laboratory study

• DOI: 10.1007/s12020-019-01937-6
• Publication Date: 2019-04-26T05:04:21Z
• Authors: Jeffery S. Staab; Tracey J. Smith; Marques Wilson; Scott J. Montain; Erin Gaffney-Stom...
• Keywords: Adult; Male; 0301 basic medicine; Time Factors; Osteocalcin; Alkaline Phosphatase; Collagen Type I; Young Adult; 03 medical and health sciences; Bone Density; Parathyroid Hormone; Case-Control Studies; Humans; Sleep Deprivation; Calcium; Bone Remodeling; Bone Resorption; Sleep; Biomarkers

The objective of the study was to evaluate how controlled, short-term sleep restriction (SR; 72 h) alters markers of bone formation and resorption and urinary calcium (Ca) output.Ten healthy, sleep-adequate, male soldiers were housed in the research facility one day prior to and for the duration of SR. Diet was controlled to provide adequate energy balance and macronutrient distribution, meeting the recommended dietary allowance (RDA) for Ca. Subjects engaged in light activities to maintain wakefulness and were allowed 2 h of sleep per night (0430-0630 hours). Blood samples were collected each morning at 0 h (baseline) and 24, 48, and 72 h of SR. Serum was assayed for parathyroid hormone (PTH), bone alkaline phosphatase (BAP), tartrate-resistant acid phosphatase (TRAP), and C-terminal telopeptide of type I collagen (CTX). Urine was collected in 24 h increments during SR for measurement of Ca and creatinine (Cr).BAP was reduced at 24 h (P= 0.015) and resorption markers TRAP and CTX were increased after 48 and 72 h of SR compared to baseline (P < 0.05). The ratio of BAP:TRAP was significantly lower (P= 0.017) at 48 and 72 h of SR. In contrast, total 24 h urinary Ca and Ca/Cr excretion were unchanged.Markers of bone formation and resorption are uncoupled in response to as little as 48 h of SR even when Ca intake is at the RDA. Sleep deprivation may be a risk factor for reduced bone health due to perturbations in bone turnover.