Short-Lived Human Umbilical Cord-Blood-Derived Neural Stem Cells Influence the Endogenous Secretome and Increase the Number of Endogenous Neural Progenitors in a Rat Model of Lacunar Stroke

• DOI: 10.1007/s12035-015-9530-6
• Publication Date: 2015-11-25T10:43:24Z
• Authors: Anna Jablonska; Katarzyna Drela; Luiza Wojcik-Stan...; Miroslaw Janowski; Teresa Zalewska; Barbara Lukomska
• Keywords: Doublecortin Domain Proteins; Male; Proteome; Cell Survival; Neurogenesis; Neuropeptides; Brain; Cell Count; Fetal Blood; Article; Matrix Metalloproteinases; 3. Good health; Cellular and Molecular Neuroscience; Disease Models, Animal; 03 medical and health sciences; 0302 clinical medicine; Bromodeoxyuridine; Neural Stem Cells; Animals; Humans; Nerve Growth Factors; RNA, Messenger; Ouabain; Microtubule-Associated Proteins; Cell Proliferation

Stroke is the leading cause of severe disability, and lacunar stroke related to cognitive decline hemiparesis. There no effective treatment for majority patients with stroke. Thus, stem cell-based regenerative medicine has drawn a growing body attention due capabilities trophic factor expression neurogenesis enhancement. Moreover, it was shown in an experimental autoimmune encephalomyelitis (EAE) model that even short-lived cells can be therapeutic, we have previously observed phenomenon indirectly. Here, rat stroke, investigated molecular mechanisms underlying positive therapeutic effects human umbilical cord-blood-derived neural (HUCB-NSCs) through distinct measurement exogenous endogenous factors. We also evaluated metalloproteinase activity as cellular components activity. As expected, increased proliferation migration progenitors, well up 14 days post transplantation. These changes were most prominent at 7-day time point when 30 % increases number bromodeoxyuridine (BrdU)-positive HUCB-NSC transplanted animals. The factors present until 7 transplantation, which correlated survival graft. For these days, level messenger RNA (mRNA) analyzed from 300-fold CNTF 10,000-fold IGF, much higher compared constitutive HUCB-NSCs vitro. What interesting there increase during graft survival, non-transplanted However, prolongation period while, animals, significant drop point. conclude effect may net amount (rat + human) death prolonged subsequently.