The Effect of Sleep Deprivation and Subsequent Recovery Period on the Synaptic Proteome of Rat Cerebral Cortex

• DOI: 10.1007/s12035-021-02699-x
• Publication Date: 2022-01-05T23:02:43Z
• Authors: Péter Gulyássy; Katalin Todorov-V...; Vilmos Tóth; Balázs A. Györffy; Gina Puska; Attila Simor; Gábor Juhász; László Drahos; Katalin Adrienna ...
• Keywords: Proteome; Synaptosome; Postsynaptic density; Cognitive Decline; Sleep

Sleep deprivation (SD) is commonplace in the modern way of life and has a substantial social, medical, human cost. induces cognitive impairment such as loss executive attention, working memory decline, poor emotion regulation, increased reaction times, higher functions are particularly vulnerable to sleep loss. Furthermore, SD associated with obesity, diabetes, cardiovascular diseases, cancer, vast majority psychiatric neurodegenerative disorders accompanied by disturbances. Despite widespread scientific interest effect on synaptic function, there lack investigation focusing transmission proteome level. In present study, we report effects recovery period (RP) cortical rats. Synaptosomes were isolated after 8 h performed gentle handling 16 RP. The purity synaptosome fraction was validated western blot electron microscopy, protein abundance alterations analyzed mass spectrometry. We observed that RP have wide impact neurotransmitter-related proteins at both presynaptic postsynaptic membranes. changed greater extent consequence than during RP: identified 78 altered 39 course Levels most upregulated SD, while showed opposite tendency, three (Gabbr1, Anks1b, Decr1) changes direction functional cluster analysis revealed related signal transduction assembly, ion transport, lipid fatty acid metabolism, interaction network several connections between significantly molecular processes plasticity or sleep. Our proteomic data implies suppression SNARE-mediated vesicle exocytosis impaired endocytic deprivation. Both GABA neurotransmission affected synthesis, regulatory signaling pathways, energy homeostatic processes, metabolic pathways.