Ginsenoside Rg1 Reduced Microglial Activation and Mitochondrial Dysfunction to Alleviate Depression-Like Behaviour Via the GAS5/EZH2/SOCS3/NRF2 Axis

• DOI: 10.1007/s12035-022-02740-7
• Publication Date: 2022-03-01T16:41:50Z
• Authors: Junnan Li; Wei Gao; Zhonghui Zhao; Yannan Li; Lixuan Yang; Wei Wei; Feifei Ren; Yang Li; Yao Yu; Wenzhe Duan; Jingchun Li; Baoan Dai; Rongjuan Guo
• Keywords: GAS5

Ginsenoside Rg1 is the principal active ingredient in ginseng. The antidepressant effects of have been validated; however, specific underlying mechanism this effect needs further research. Rats were subjected to chronic restraint stress (CRS) depression model. Rg1, or a positive control drug, was administered rats. Depression-like behaviours evaluated through behavioural experiments. Cytokine, mRNA, protein, ATP, and mitochondria DNA levels detected using indicated methods. Lentivirus-packaged plasmids injected into rat brain for GAS5 overexpression knockdown. In vitro mitochondrial dysfunction by detecting reactive oxygen species membrane potential. Direct interaction between EZH2 validated RNA immunoprecipitation pull-down assay. enrichment H3K27me3 chromatin quantitative real-time PCR. treatment alleviated depression-like behaviours, microglial activation, CRS Similarly, knockdown revealed similar protective treatment. compromised Moreover, attenuated activation vitro. Mechanically, suppressed SOCS3 NRF2 expression facilitating EZH2-mediated transcriptional repression. improved downregulating expression. might regulate via epigenetic suppression SOCS3.