A Single-Cell Transcriptomic Analysis of the Mouse Hippocampus After Voluntary Exercise

• DOI: 10.1007/s12035-023-03869-9
• Publication Date: 2024-01-13T06:02:00Z
• Authors: Aditi Methi; Md Rezaul Islam; Lalit Kaurani; M Sadman Sakib; Dennis M. Krüger; Tonatiuh Pena; Susanne Burkhardt; David Liebetanz; André Fischer
• Keywords: Environmental Enrichment

Exercise has been recognized as a beneficial factor for cognitive health, particularly in relation to the hippocampus, vital brain region responsible learning and memory. Previous research demonstrated that exercise-mediated improvement of memory humans rodents correlates with increased adult neurogenesis processes related enhanced synaptic plasticity. Nevertheless, underlying molecular mechanisms are not fully understood. With aim further elucidate these mechanisms, we provide comprehensive dataset mouse hippocampal transcriptome at single-cell level after 4 weeks voluntary wheel-running. Our analysis provides number interesting observations. For example, results suggest exercise affects by accelerating maturation subpopulation Prdm16-expressing neurons. Moreover, uncover existence an intricate crosstalk among multiple signaling pathways such NF-κB, Wnt/β-catenin, Notch, retinoic acid (RA) altered upon specific cluster excitatory neurons within Cornu Ammonis (CA) hippocampus. In conclusion, our study important resource sheds light on changes induced These findings have implications developing targeted interventions aimed optimizing health preventing age-related decline.