Electrochemical and spectroscopic studies of the interaction of (+)-epicatechin with bovine serum albumin

• DOI: 10.1007/s12039-021-01894-6
• Publication Date: 2021-04-25T02:44:32Z
• Authors: Jules-Blaise Mabo...; Martin Pengou; Francis Merlin Me...; Sergeot Delor Kun...; Achille Nassi; Charles Péguy Nan...; Emmanuel Ngameni
• Keywords: 0301 basic medicine; 03 medical and health sciences; 01 natural sciences; 0104 chemical sciences

The mechanism of the electrochemical oxidation of (+)-epicatechin (epiCAT) in a hydro-alcoholic medium, at a glassy carbon electrode, was studied using cyclic and square wave voltammetric techniques and at different pHs. It proceeds in a cascade mechanism related to the two resorcinol hydroxyl groups and the other three hydroxyl groups in epiCAT which present electroactivity and is pH-dependent. The oxidation of the 3′,4′-dihydroxyl moiety, occurs first at a very low positive potential, and it is a two-electron/two proton reversible reaction. The product obtained from the oxidation of the 3′,4′-dihydroxyl moiety then undergoes a chemical reaction followed by an electrochemical process to give an electroinactive product. The proposed mechanism is an ECE type mechanism. After the addition of bovine serum albumin (BSA) to epiCAT solution, the oxidation peak currents decreased with no peak potential shift and no new peaks appeared. The diffusion coefficients of both free and bound epiCAT were estimated from the cyclic voltammetry data (Df = 2.37 × 10−10 cm2s−1 and Db = 6.28 × 10−11 cm2s−1). An epiCAT-BSA complex is formed with binding constant Kapp = 1.8 × 104, calculated from UV-vis spectroscopy data. The oxidation process of (+)-epicatechin (I to I’ and II) in a hydro-alcoholic medium at a glassy carbon electrode follows an ECE type mechanism.