Heat shock protein expression analysis in canine osteosarcoma reveals HSP60 as a potentially relevant therapeutic target

• DOI: 10.1007/s12192-013-0414-2
• Publication Date: 2013-03-05T11:53:01Z
• Authors: Gayathri T. Selva...; Floor A.S. Bonestroo; Jolle Kirpensteijn; Marja J.L. Kik; Ruurd van der Zee; Willem van Eden; Elpetra P.M. Timm...; Adri Slob; Jan A. Mol
• Keywords: HSP60

Heat shock proteins (HSP) are highly conserved across eukaryotic and prokaryotic species. These play a role in response to cellular stressors, protecting cells from damage facilitating recovery. In tumor cells, HSPs can have cytoprotective effects interfere with apoptotic cascades. This study was performed assess the prognostic predictive values of gene expression HSP family members canine osteosarcoma (OS) their potential for targeted therapy. Gene expressions were assessed using quantitative PCR (qPCR) on 58 snap-frozen primary OS tumors related clinic-pathological parameters. A significant increased HSP60 found relation shorter overall survival an osteoblastic phenotype. Therefore, function investigated more detail. Immunohistochemical analysis revealed heterogeneous staining tumors. The highest immunoreactivity short surviving dogs. Next shown variety human cell lines by qPCR Western blot. two metastatic silenced siRNA resulting decreased proliferation induction apoptosis both lines. It is concluded that overexpression associated poor prognosis should be evaluated as new target