Lipocalin-2-mediated upregulation of various antioxidants and growth factors protects bone marrow-derived mesenchymal stem cells against unfavorable microenvironments

• DOI: 10.1007/s12192-013-0430-2
• Publication Date: 2013-05-01T11:06:13Z
• Authors: Raheleh Halabian; Hossein Abdul Teh...; Ali Jahanian-Naja...; Mehryar Habibi Ro...
• Keywords: 0301 basic medicine; 0303 health sciences; Superoxide Dismutase; Bone Marrow Cells; Cell Differentiation; Mesenchymal Stem Cells; Hep G2 Cells; Antioxidants; Lipocalins; Rats; Up-Regulation; Rats, Sprague-Dawley; 03 medical and health sciences; Cellular Microenvironment; Lipocalin-2; Proto-Oncogene Proteins; Cell Adhesion; Animals; Humans; Intercellular Signaling Peptides and Proteins; Heme Oxygenase-1; Acute-Phase Proteins; Cell Proliferation

Despite many advantages of mesenchymal stem cells (MSCs) that make them suitable for cell therapy purposes, their therapeutic application has been limited due to susceptibility several stresses (e.g., nutrient-poor environment, oxidative stress, and hypoxic masses cytotoxic factors) which they are exposed during preparation following transplantation. Hence, reinforcing MSCs against these is a challenge both basic clinician scientists. Recently, much attention directed toward equipping with cytoprotective factors strengthen unfavorable microenvironments. Here, we engineered lipocalin 2 (Lcn2), factor naturally induced exposure imposed by the microenvironment. Lcn2 overexpression not only did interfere multidifferentiation capacity but also granted protective properties them. potentiated withstand oxidative, hypoxia, serum deprivation (SD) conditions via antagonizing cytotoxicity apoptosis. Adhesion rate coated culture plates was enhanced overexpression. In addition, antioxidants upregulated some growth in MSCs. Our findings suggested new strategy prevention graft death MSC-based therapy.