Seasonal variation in expression pattern of genes under HSP70

• DOI: 10.1007/s12192-013-0469-0
• Publication Date: 2013-10-11T17:07:30Z
• Authors: Dipak Banerjee; Ramesh C. Upadhyay; Umesh B. Chaudhary; Ravindra Kumar; Sohanvir Singh; Ashutosh; Jagan Mohanarao G.; Shamik Polley; Ayan Mukherjee; Tapan K. Das; Sachinandan De
• Keywords: 2. Zero hunger; 0301 basic medicine; 0303 health sciences; DNA, Complementary; Hot Temperature; Climate; Gene Expression Profiling; Goats; Real-Time Polymerase Chain Reaction; Adaptation, Physiological; Cold Temperature; 03 medical and health sciences; Gene Expression Regulation; 13. Climate action; Sequence Homology, Nucleic Acid; Animals; Female; HSP70 Heat-Shock Proteins; Seasons; Phylogeny

Heat shock protein 70 (HSP70) is one of the most abundant and best characterized heat shock protein family that consists of highly conserved stress proteins, expressed in response to stress, and plays crucial roles in environmental stress tolerance and adaptation. The present study was conducted to identify major types of genes under the HSP70 family and to quantify their expression pattern in heat- and cold-adapted Indian goats (Capra hircus) with respect to different seasons. Five HSP70 gene homologues to HSPA8, HSPA6, HSPA1A, HSPA1L, and HSPA2 were identified by gene-specific primers. The cDNA sequences showed high similarity to other mammals, and proteins have an estimated molecular weight of around 70 kDa. The expression of HSP70 genes was observed during summer and winter. During summer, the higher expression of HSPA8, HSPA6, and HSPA1A was observed, whereas the expression levels of HSPA1L and HSPA2 were found to be lower. It was also observed that the expression of HSPA1A and HSPA8 was higher during winter in both heat- and cold-adapted goats but downregulates in case of other HSPs. Therefore, both heat and cold stress induced the overexpression of HSP70 genes. An interesting finding that emerged from the study is the higher expression of HSP70 genes in cold-adapted goats during summer and in heat-adapted goats during winter. Altogether, the results indicate that the expression pattern of HSP70 genes is species- and breed-specific, most likely due to variations in thermal tolerance and adaptation to different climatic conditions.