Involvement of small heat shock proteins, trehalose, and lipids in the thermal stress management in Schizosaccharomyces pombe

• DOI: 10.1007/s12192-015-0662-4
• Publication Date: 2015-12-02T21:45:47Z
• Authors: Attila Glatz; Ana-Maria Pilbat; Gergely L. Németh; Katalin Vince-Kontár; Katalin Jósvay; Ákos Hunya; Andor Udvardy; Imre Gombos; Mária Péter; Gábor Balogh; Ibolya Horváth; László Vígh; Zsolt Török
• Keywords: 0303 health sciences; Hot Temperature; QH3011 Biochemistry / biokémia; Lipid Bilayers; Trehalose; Lipid Metabolism; Heat-Shock Proteins, Small; 03 medical and health sciences; Stress, Physiological; Gene Expression Regulation, Fungal; Mutation; Schizosaccharomyces; Schizosaccharomyces pombe Proteins

Changes in the levels of three structurally and functionally different important thermoprotectant molecules, namely small heat shock proteins (sHsps), trehalose, lipids, have been investigated upon Schizosaccharomyces pombe. Both α-crystallin-type sHsps (Hsp15.8 Hsp16) were induced after prolonged high-temperature treatment but with kinetic profiles. The shsp null mutants display a weak, significant, sensitivity indicating their importance thermal stress management. induction is wild type highly heat-sensitive trehalose-deficient (tps1Δ) cells; however, trehalose level did not show significant alteration mutants. altered timing accumulation suggest that disaccharide might provide protection at early stage while elevated amount are required later phase. cellular lipid compositions two temperature-adapted wild-type S. pombe cells also according to rule homeoviscous adaptation, crucial role adapting environmental temperature changes. Hsp15.8 Hsp16 able bind lipids isolated from pombe, whose interaction powerful against heat-induced damages membranes. Our data all macromolecules play pivotal during management fission yeast.