Tanshinone IIA protects mouse testes from heat stress injury by inhibiting apoptosis and TGFβ1/Smad2/Smad3 signaling pathway

• DOI: 10.1007/s12192-023-01367-4
• Publication Date: 2023-08-23T09:29:09Z
• Authors: Lin Bai; Yaping Zhang; Changmin Zheng; Shifu Xu; Yining He; Guangqiang Yu; Delun Huang; Yulin Huang; Mingxing Li; Changlong Xu
• Keywords: Male; Mice; Testis; Animals; Apoptosis; Testosterone; Heat-Shock Response; 3. Good health; Signal Transduction

Heat stress can cause testicular damage and affect male fertility. Tanshinone IIA (TSA) is a monomer substance derived from plants, with antioxidant anti-apoptotic effects. Whether it repair caused by heat unclear. This study aims to construct mouse injury model intervene TSA. Various methods such as histopathology, high-throughput sequencing, bioinformatics analysis, molecular biology were used investigate whether TSA alleviate stress-induced its mechanism. Results showed that significantly reduced the diameter of seminiferous tubules, increased cell apoptosis in tissue, decreased testosterone levels. After intervention, morphology improved significantly, secretion function was restored. High-throughput transcriptome sequencing found key differentially expressed genes between HS group control groups clustered TGFβ signaling pathways. Using western blot technology, we upregulated TGFβ1/Smad2/Smad3 pathway protein expression, causing apoptosis, tissue organic lesions, affecting function. Through inhibit thereby restoring stress. confirms effectively restore mice, possibly inhibiting suppress apoptosis.