Expression patterns of Ruminococcus flavefaciens 007S cellulases as revealed by zymogram approach

• DOI: 10.1007/s12223-012-0144-3
• Publication Date: 2012-04-12T07:19:00Z
• Authors: Maša Vodovnik; Romana Marinšek L...
• Keywords: Ruminococcus; Zymography

The expression of Ruminococcus flavefaciens 007S cellulases in different incubation time points (growth stages) and their substrate inducibility were analyzed by comparing the zymogram expression profiles of cultures grown on insoluble cellulose (Avicel) with cellobiose-grown cultures. The molecular weights of the enzymes were compared to (putative) cellulases encoded in the R. flavefaciens FD-1 genome.