A New RNA-Seq Method to Detect the Transcription and Non-coding RNA in Prostate Cancer

• DOI: 10.1007/s12253-013-9618-0
• Publication Date: 2013-09-16T15:50:35Z
• Authors: Xiao-Ming Zhang; Zhong-Wei Ma; Qiang Wang; Jian-Ning Wang; Ji-Wei Yang; Xian-Duo Li; Hao Li; Tong-Yi Men
• Keywords: RNA-Seq; Transcription

Prostate cancer is a big killer in many regions especially American men, and this year, the diagnosed rate rises rapidly. We aimed to find the biomarker or any changing in prostate cancer patients. With the development of next generation sequencing, much genomic alteration has been found. Here, basing on the RNA-seq result of human prostate cancer tissue, we tried to find the transcription or non-coding RNA expressed differentially between normal tissue and prostate cancer tissue. 10 T sample data is the RNA-seq data for prostate cancer tissue in this study, we found the differential gene is TFF3-Trefoil factor 3, which was more than seven fold change from prostate cancer tissue to normal tissue, and the most outstanding transcript is C15orf21. Additionally, 9 lncRNAs were found according our method. Finally, we found the many important non-coding RNA related to prostate cancer, some of them were long non-coding RNA (lncRNA).