Systemic Delivery of Nanoparticles Loaded with Pentagalloyl Glucose Protects Elastic Lamina and Prevents Abdominal Aortic Aneurysm in Rats

• DOI: 10.1007/s12265-016-9709-x
• Publication Date: 2016-08-19T09:25:46Z
• Authors: Nasim Nosoudi; Aniqa Chowdhury; Steven Siclari; Vaideesh Parasaram; Saketh Karamched; Naren Vyavahare
• Keywords: Male; 0303 health sciences; Drug Compounding; Macrophages; Elastic Tissue; Antibodies; Hydrolyzable Tannins; Matrix Metalloproteinases; Elastin; 3. Good health; Calcium Chloride; Disease Models, Animal; Drug Liberation; 03 medical and health sciences; Cytoprotection; Injections, Intravenous; Proteolysis; Animals; Nanoparticles; Aorta, Abdominal; Particle Size; Cells, Cultured; Aortic Aneurysm, Abdominal

Degeneration of elastin plays a vital role in the pathology and progression of abdominal aortic aneurysm (AAA). Our previous study showed that pentagalloyl glucose (PGG), a core derivative of tannic acid, hinders the development of AAAs in a clinically relevant animal model when applied locally. In this study, we tested whether targeted nanoparticles (NPs) can deliver PGG to the site of an aneurysm and prevent aneurysmal growth by protecting elastin. PGG-loaded albumin NPs with a surface-conjugated elastin-specific antibody were prepared. Aneurysms were induced by calcium chloride-mediated injury to the abdominal aorta in rats. NPs were injected into the tail vein after 10 days of CaCl2 injury. Rats were euthanized after 38 days. PGG delivery led to reduction in macrophage recruitment, matrix metalloproteinase (MMP) activity, elastin degradation, calcification, and development of aortic aneurysm. Such NP delivery offers the potential for the development of effective and safe therapies for AAA.