Chemical constituents of Acanthus ilicifolius L. and effect on osteoblastic MC3T3E1 cells

• DOI: 10.1007/s12272-001-1232-3
• Publication Date: 2008-08-13T06:21:53Z
• Authors: Phan Van Kiem; Tran Hong Quang; Tran Thu Huong; Le Thi Hong Nhung; Nguyen Xuan Cuong; Chau Van Minh; Eun Mi Choi; Young Ho Kim
• Keywords: 0301 basic medicine; Magnetic Resonance Spectroscopy; Osteoblasts; Bone Density Conservation Agents; Chemical Phenomena; Spectrophotometry, Infrared; Chemistry, Physical; Plant Extracts; Hydrolysis; Molecular Conformation; Tetrazolium Salts; Cell Differentiation; 3T3 Cells; Alkaline Phosphatase; Mass Spectrometry; Plant Leaves; Mice; 03 medical and health sciences; Acanthaceae; Animals; Calcium; Collagen

A new coumaric acid derivative called acancifoliuside (1) and six known compounds as acteoside (2), isoacteoside (3), acanthaminoside (4), (+)-lyoniresinol 3a-O-beta-glucopyranoside (5), (-)-lyoniresinol (6), and alpha-amyrin (7), were isolated from the methanolic extract of the leaves of Acanthus ilicifolius L. (Acanthaceae). Their structures were determined by spectroscopic methods and a comparison with the spectral data reported in the literature. The effects of the compounds on the function of osteoblastic MC3T3-E1 cells were tested. Compounds 2, 3, and 5 (30 microM) increased the growth and differentiation of osteoblasts significantly (P<0.05), indicating that A. ilicifolius leaves may help prevent osteoporosis.