Toxicity and tissue distribution of cerium oxide nanoparticles in rats by two different routes: single intravenous injection and single oral administration

• DOI: 10.1007/s12272-018-1074-7
• Publication Date: 2018-09-03T07:41:39Z
• Authors: Kwangsik Park; Juyoung Park; Handule Lee; Jonghye Choi; Wook-Joon Yu; Jinsoo Lee
• Keywords: 0301 basic medicine; No-Observed-Adverse-Effect Level; Dose-Response Relationship, Drug; Metabolic Clearance Rate; Administration, Oral; Cerium; 6. Clean water; 3. Good health; Rats, Sprague-Dawley; 03 medical and health sciences; Liver; Injections, Intravenous; Toxicity Tests; Animals; Nanoparticles; Female; Tissue Distribution; Particle Size; Spleen

Toxicity and target organ distribution of cerium oxide nanoparticles (CeNPs) were investigated via single intravenous injection and single oral administration, respectively. Rats were sacrificed at 24 h after treatment with doses of 30 and 300 mg/kg, and cerium concentrations were measured in liver, kidney, spleen, lung, blood, urine and feces. Results revealed cerium levels in blood and tissues were considerably low in oral treated groups and most cerium was detected in feces, meaning CeNPs would not be absorbed in the gastro-intestinal system. Conversely, high concentrations of cerium were detected in all tissues of rats after intravenous injection. Liver and spleen were main target organs. Cerium levels in liver were 594.9 ± 95.3 μg/g tissue in 30 mg/kg treat group and 3741.7 ± 932.7 μg/g tissue in 300 mg/kg treat group. Cerium levels in spleen reached almost levels of liver. Cerium was also detected, that is relatively low compared to oral administration, in feces of rats treated via intravenous injection, that supports biliary excretion of CeNPs. Urine excretion of CeNPs was not detected in oral treatment and intravenous injection. In accordance with level of cerium distribution, toxicities based on hematology, serum biochemistry and histopathology were observed in rats treated by intravenous injection while no significance was revealed in orally treated groups.