Pullulan acetate coated magnetite nanoparticles for hyper-thermia: Preparation, characterization and in vitro experiments

• DOI: 10.1007/s12274-010-1004-6
• Publication Date: 2010-01-17T21:39:52Z
• Authors: Fuping Gao; Yuanyuan Cai; Jing Zhou; Xiaoxue Xie; Weiwei Ouyang; Yuhui Zhang; Xufei Wang; Xiaodong Zhang; Xiaowen Wang; Lingyun Zhao; Jintian Tang
• Keywords: Superparamagnetism; Pullulan; Magnetic hyperthermia; Thermogravimetric analysis

Amphipathic polymer pullulan acetate (PA)-coated magnetic nanoparticles were prepared and characterized by various physicochemical means. The cytotoxicity cellular uptake of the examined. hyperthermic effect on tumor cells was evaluated. Transmission electron microscopy (TEM) showed that PA coated (PAMNs) had spherical morphology. Dynamic light scattering (DLS) size distribution PAMNs unimodal,with an average diameter 25.8 nm ± 6.1 nm. presence adsorbed layer magnetite surface confirmed Fourier transform infrared (FTIR) spectroscopy. Magnetic measurements revealed saturation magnetization reached 51.9 emu/g superparamagnetic. Thermogravimetric analysis (TGA) Fe3O4 particles constituted 75 wt% PAMNs. good heating properties in alternating field. Cytotoxicity assay exhibited no significant against L929 cells. TEM results a large number internalized into KB have hyperthermia vitro field induced hyperthermia. These novel great potential as mediators.