Isolation and characterization of a novel chlorpyrifos degrading flavobacterium species EMBS0145 by 16S rRNA gene sequencing

• DOI: 10.1007/s12539-012-0207-9
• Publication Date: 2014-09-23T19:37:17Z
• Authors: P. Amareshwari; Mayuri Bhatia; K. Venkatesh; A. Roja Rani; G. V. Ravi; Priyanka Bhakt; Srinivas Bandaru; Mukesh Yadav; Anuraj Nayarisseri; Achuthsankar S. Nair
• Keywords: DNA, Bacterial; 0301 basic medicine; RNA, Bacterial; 03 medical and health sciences; Biodegradation, Environmental; RNA, Ribosomal, 16S; Molecular Sequence Data; Chlorpyrifos; Sequence Analysis, DNA; Pesticides; Flavobacterium; Phylogeny

Indiscriminate application of pesticides like chlorpyrifos, diazinon, or malathion contaminate the soil in addition has being unsafe often it has raised severe health concerns. Conversely, microorganisms like Trichoderma, Aspergillus and Bacteria like Rhizobium Bacillus, Azotobacter, Flavobacterium etc have evolved that are endowed with degradation of pesticides aforementioned to non-toxic products. The current study pitches into identification of a novel species of Flavobacterium bacteria capable to degrade the Organophosphorous pesticides. The bacterium was isolated from agricultural soil collected from Guntur District, Andhra Pradesh, India. The samples were serially diluted and the aliquots were incubated for a suitable time following which the suspected colony was subjected to 16S rDNA sequencing. The sequence thus obtained was aligned pairwise against Flavobacterium species, which resulted in identification of novel specie of Flavobacterium later named as EMBS0145, the sequence of which was deposited in in GenBank with accession number JN794045.