Expressed sequence tag analysis of phyllosomas and hemocytes of Japanese spiny lobster Panulirus japonicus

• DOI: 10.1007/s12562-008-0027-3
• Publication Date: 2009-02-18T23:06:22Z
• Authors: Duangjai Pisuttha...; Motoshige Yasuike; Hideaki Aono; Keisuke Murakami; Hidehiro Kondo; Takashi Aoki; Ikuo Hirono
• Keywords: 0301 basic medicine; 03 medical and health sciences

Two complementary DNA (cDNA) libraries were constructed from phyllosomas and hemocytes of adult Japanese spiny lobster Panulirus japonicus and a total of 2,673 expressed sequence tags (ESTs) were obtained. After assembly and clustering, 450 and 458 unique sequences were found from the phyllosoma and hemocyte cDNA libraries, respectively. Of these, 114 and 220 ESTs showed significant homologies with known genes in the National Centre for Biotechnology Information (NCBI) database. The remaining sequences were of unknown function. Immune-related genes found in this study include lectin, proteinase inhibitor, prophenoloxidase, heat-shock protein, antimicrobial peptide, and a few putative defense-related proteins.