Antibacterial, Antioxidant, and Anti-inflammatory Activities of Lacticaseibacillus paracasei Lysates Isolated from Fermented Palm Sap

• DOI: 10.1007/s12602-025-10521-6
• Publication Date: 2025-03-23T06:55:34Z
• Authors: Phoomjai Sornsenee; Nateelak Kooltheat; Dechawat Wongprot; Pinkanok Suksabay; Tae-Gyu Nam; Uttapol Permpoon; Phanvasri Saengsuwan; Chonticha Romyasamit
• Keywords: Anti-inflammatory

Paraprobiotics are inactivated microbial cells or cell fractions that confer health benefits to the consumer, and possess ability regulate both adaptive innate immune systems. They exhibit anti-inflammatory, antiproliferative, antioxidant properties, antimicrobial activities. The aim of this study is evaluate these activities Lacticaseibacillus paracasei lysates isolated from fermented palm sap. bacterial were prepared via sonication, their antibacterial activity was assessed against three pathogens using agar well diffusion assay. Antioxidant evaluated DPPH ABTS radical scavenging assays. Cytotoxicity anti-inflammatory determined in LPS-stimulated RAW 264.7 by measuring nitric oxide production, secretion, mRNA level cytokines (IL-1β, IL-6, IL-10). Among lysates, L. T1901 demonstrated strongest Escherichia coli, Bacillus cereus, Acinetobacter baumannii. Most exhibited potent activity, especially T0601 showed highest In cells, effectively reduced levels suppressed production pro-inflammatory (IL-1β IL-6); simultaneously, enhanced immunosuppressive cytokine IL-10 secretion. Furthermore, no cytotoxic effect observed lysate-treated cells. highlights potential as multifunctional agents with antibacterial, antioxidant, properties. These findings support application developing functional foods therapeutic for managing oxidative stress inflammatory diseases.