The role of the ALKBH5 RNA demethylase in invasive breast cancer

• DOI: 10.1007/s12672-024-01205-8
• Publication Date: 2024-08-11T07:01:51Z
• Authors: Corinne L. Woodcock; Mansour Alsaleem; Michael S. Toss; Jennifer Lothion-Roy; Anna E. Harris; Jennie N. Jeyapalan; Nataliya Blatt; Albert A. Rizvanov; Regina R. Miftakhova; Yousif A. Kariri; Srinivasan Madhus...; Andrew R. Green; Catrin S. Rutland; Rupert G. Fray; Emad A. Rakha; Nigel P. Mongan
• Keywords: Demethylase; Molecular Pathology

Abstract Background N6-methyladenosine (m 6 A) is the most common internal RNA modification and involved in regulation of protein expression. AlkB family member 5 (ALKBH5) a m A demethylase. Given important role biological mechanisms, its regulators, have been implicated many disease processes, including cancer. However, contribution ALKBH5 to invasive breast cancer (BC) remains poorly understood. The aim this study was evaluate clinicopathological value BC. Methods Publicly available data were used investigate mRNA alterations, prognostic significance, association with clinical parameters at genomic transcriptomic level. Differentially expressed genes (DEGs) enriched pathways low or high expression investigated. Immunohistochemistry (IHC) assess large well-characterised BC series (n = 1327) determine significance Results Reduced significantly associated poor prognosis unfavourable parameters. gene harboured few mutations and/or copy number alternations, but seen. Patients had differentially pathways, cytokine-cytokine receptor interaction pathway. Low tumour progression larger size worse Nottingham Prognostic Index group. Conclusion This implicates highlights need for further functional studies decipher methylation progression.