Unravelling the role of PRKCI and key-cancer related genes in breast cancer development and metastasis

• DOI: 10.1007/s12672-025-02133-x
• Publication Date: 2025-03-18T15:04:25Z
• Authors: Hania Shah; Khushbukhat Khan; Yasmin Badshah; Janeen H. Trembley; Naeem Mahmood Ashraf; Maria Shabbir; Tayyaba Afsar; Dara Aldisi; Dilawer Khan; Suhail Razak
• Keywords: Breast cancer metastasis

Breast cancer is one of the most common causes fatalities in females globally. Rising cases drug resistance against existing chemotherapeutics are great problem. To address this issue, there a need to find appropriate biomarker that could be used detect at early stages, so development can avoided. Protein Kinase C iota (PKCɩ), an AGC kinase, has oncogenic role cancers and its expression Single nucleotide polymorphisms (SNPs) have been reported associated with development. So, study aims were examine PKCɩ, B (AKT), Suppressor cytokine signaling 3 (SOCS3), Vascular endothelial growth factor (VEGF), Krupple like (KLF3), Tumor protein D52 (TPD52), Hypoxia inducible (HIF1α) microRNA-124 (miR-124) breast association PKCɩ variants (G34W & F66Y) cancer. Genetic assay was performed through real time Polymerase Chain reaction (PCR), whereas genotypic SNPs accomplished Tetra-ARMS PCR. The levels AKT, SOC3, VEGF, HIF1α TPD52 elevated patients as compared control miR-124 KLF3 lowered patients. Positive variant G34W (TT) explored ARM's PCR, while no F66Y found. Hence, results suggest related genes after further verification serve potential biomarkers for early-diagnosis prognosis