Microsatellite marker development and putative SNP detection for a northward expanding damselfly species using next generation sequencing

• DOI: 10.1007/s12686-012-9711-9
• Publication Date: 2012-07-14T10:05:38Z
• Authors: J. Swaegers; J. Mergeay; G. E. Maes; J. K. J. Van Houdt; M. H. D. Larmuseau; R. Stoks
• Keywords: 0303 health sciences; 03 medical and health sciences

In this study we used Roche’s 454 sequencing to develop genetic markers for Coenagrion scitulum, a Mediterranean damselfly currently expanding its range northward. With a modest amount of sequencing we detected 6,318 potential microsatellite markers and 832 putative single nucleotide polymorphisms (SNPs). From the potential microsatellite markers we developed thirteen novel microsatellite markers. Among other applications, these markers can be used to unravel the micro-evolutionary consequences of range expansion in this species.