A multiplex microsatellite set for non-invasive genotyping and sexing of the osprey (Pandion haliaetus)

• DOI: 10.1007/s12686-015-0497-4
• Publication Date: 2015-10-29T09:07:44Z
• Authors: Deborah A. Dawson; Oddmund Kleven; Natalie dos Remedios; Gavin J. Horsburgh; Rolf T. Kroglund; Teresa Santos; Colin R. A. Hewitt
• Keywords: Simple Tandem Repeat (STR); 590; Sex-typing; Microsatellite Letters; 01 natural sciences; Raptor; Western osprey; Swabs; Feather; Birds of prey; 14. Life underwater; 0105 earth and related environmental sciences

During the 1950s and 1970s osprey (Pandion haliaetus) experienced a dramatic population crash remains of conservation concern in several parts world. We isolated 37 microsatellite loci assessed these ospreys sampled UK Norway (using mouth swabs/feathers). From 26 variable four ospreys, we selected 13, combined into two multiplex-PCR sets included sex-typing marker. Additional markers confirmed sexes. In 17 feather-sampled central Norway, found 3–10 alleles per locus. The 13 are autosomal (heterozygotes were present both sexes) observed heterozygosities ranged from 0.24 to 0.94. probability identity for was 8.0 × 10−12. These will be useful genetic monitoring, parentage analysis studies osprey.