Characterization of 55 SNP markers in Chinese longsnout catfish Leriocassis Longirostris

• DOI: 10.1007/s12686-020-01137-9
• Publication Date: 2020-02-26T05:32:33Z
• Authors: Wei Zhao; Jian Zhou; Zhe Li; Tingsen Jing; Li Zhao; Hua Ye
• Keywords: Linkage Disequilibrium; SNP; Germ plasm

The Chinese longsnout catfish (Leiocassis longirostris) is a commercially important aquaculture species in China. The germplasm resources of L. longirostris have been seriously degraded by human activities, therefore, it is very urgent to develop genetic markers of L. longirostris to investigate the natural resources. Here, a total of 55 single nucleotide polymorphism (SNP) loci were identified using Sequenom MassARRAY based on the L. longirostris transcriptome. Genetic parameters of the loci showed that the minor allele frequency varied from 0.0417 to 0.5000, and that the observed and expected heterozygosity ranged from 0.0833 to 0.8750 and 0.0802 to 0.5021, respectively. 27 loci were identified deviating significantly from the Hardy–Weinberg equilibrium (P < 0.05), and no linkage disequilibrium was found between pairs of loci. These SNP markers will be useful for future population conservation and construction of genetic linkage maps related to L. longirostris.