Enzymatic production of glucosamine and chitooligosaccharides using newly isolated exo-β-d-glucosaminidase having transglycosylation activity

• DOI: 10.1007/s13205-015-0330-5
• Publication Date: 2016-01-06T08:20:53Z
• Authors: Sujata Sinha; Subhash Chand; Pushplata Tripathi
• Keywords: Chitosanase; Aspergillus oryzae

Exochitosanase secreting fungus (A. fumigatus IIT-004) was isolated from fish waste using 1 % (w/v) chitosan as sole carbon source after multistage screening. Chitosan-dependent exochitosanase enzyme production (6 IU ml-1) in log phase of growth (chitosan utilization rate 0.11 g g-1 cell h-1) observed for Aspergillus minimal salt medium and there no glucose medium. Enzyme found to be extracellular subjected purification by a number steps like acetone fractionation well column chromatography. 40 yield 26-fold achieved all the steps. Purified characterized optimum temperature, pH, ionic strength substrate specificity. The K m V max purified calculated 8 mg ml-1 5.2 × 10-6 mol mg-1 min-1. immobilized on polyacrylonitrile nanofibres membrane matrix adsorption amidination. Enzymatic glucosamine various substrates free/immobilized compared. Isolated also showed transglycosylation activity.