Assessment of protein silver nanoparticles toxicity against pathogenic Alternaria solani

• DOI: 10.1007/s13205-016-0515-6
• Publication Date: 2016-09-21T07:22:25Z
• Authors: Sobhy I. I. Abdel...; Nivien A. Nafady; Ismail R. Abdel-R...; Abeer M. Shaltout; José-Antonio Daròs; Mohamed A. Mohamed
• Keywords: Alternaria solani; Silver nanoparticle; Fungal pathogen; Pathogenic fungus

Mycogenic synthesis of silver nanoparticles (AgNPs) was carried out in the present investigation using an aqueous extract endophytic non-pathogenic Alternaria solani F10 (KT721914). The mycosynthesized AgNPs were characterized by means spectroscopic and microscopic techniques. surface plasmon resonance found at 430 nm confirmed formation stable for several weeks room temperature. Also, results revealed spherical monodispersed with average size 14.8 ± 1.2 nm. FT-IR spectrum suggested that fungal extracellular proteins secondary metabolites had role Ag reduction capping which protein nanoconjugates formed. Furthermore, exhibited potent antifungal activity against different pathogenic isolates same fungus, causal pathogen tomato early blight disease. efficiency 1, 5 10 ppm evaluated 8 days after incubation measuring inhibition rate radial growth. further supported investigating hyphae morphology alteration scanning transmission electron microscopy. Treated showed pits pores. able to pass distribute throughout cell area interact components.