In vitro evaluation of anti-proliferative, anti-inflammatory and pro-apoptotic activities of the methanolic extracts of Andrographis nallamalayana Ellis on A375 and B16F10 melanoma cell lines

• DOI: 10.1007/s13205-016-0529-0
• Publication Date: 2016-10-01T08:17:53Z
• Authors: Guroji Purushotham; Yerukala Padma; Yusuf Nabiha; R. R. Venkata Raju
• Keywords: Andrographis Paniculata; Clonogenic assay; Cyclin B1; Viability assay; Andrographolide; MTT assay

Natural plant products have been widely used in controlling cancer with fewer or no side effects and the use of extracts as complementary to synthetic medicine is gaining increased popularity. Members Andrographis plants possess important medicinal properties. In present study, anti-cancerous properties nallamalayana (AN) were tested on A375 B16F10 skin melanoma cell lines. The leaf AN significantly reduced viability survival lines, achieved by MTT assay clonogenic assays, respectively. Further, TUNEL assays revealed that induces apoptosis. Western blot analysis expression Bcl-2, an anti-apoptotic protein induced proapoptotic molecules such Bcl-2 associated death promoter (BAD), X (BAX) cleaved caspase-3. Moreover, qRT-PCR western demonstrated G2/M phase proteins cdk1, cyclin B1 p53, cyclin-dependent kinase 1 inhibitor, p21. immunofluorescence NF-κB nuclear translocation, luciferase reporter gene activation. showed target genes. results concluded exhibited significant anti-proliferative, anti-inflammatory pro-apoptotic activities