Targeted DNA complementation on a 1,1′-carbonyldiimidazole-functionalized surface for identifying Mycobacterium tuberculosis

• DOI: 10.1007/s13205-020-02216-2
• Publication Date: 2020-05-02T17:02:41Z
• Authors: Shu Xu; Yu Xue; Fengyan Guo; Miaomiao Xu; Subash C. B. Gopi...; Xiaohui Mao
• Keywords: 0301 basic medicine; 03 medical and health sciences; 3. Good health

Herein, a rapid and sensitive current-volt measurement was developed for identifying the IS6110 DNA sequence to diagnose Mycobacterium tuberculosis (TB). An aminated capture probe was immobilized on a 1,1'-carbonyldiimidazole-functionalized interdigitated electrode (IDE) silica substrate, and the target sequence was detected by complementation. It was found that all tested concentrations displayed a higher response in current changes than the control, and the limit of detection was 10 fM. The sensitivity ranged from 1 to 10 fM. The control sequences with single-, triple-mismatch and noncomplementary sequences showed great discrimination. This rapid and easy DNA detection method helps to identify M. tuberculosis for early-stage diagnosis of TB.