Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis

• DOI: 10.1007/s13238-020-00770-2
• Publication Date: 2020-08-14T12:25:53Z
• Authors: Xiaojuan Liang; Cong Tao; Jianfei Pan; Lilan Zhang; Lulu Liu; Ying Zhao; Yiping Fan; Chunwei Cao; Jiali Liu; Jin Zhang; Sin Man Lam; Guanghou Shui; Wanzhu Jin; Wei Li; Jianguo Zhao; Kui Li; Yanfang Wang
• Keywords: PRDM16; Hyperinsulinemia; Thermogenin

Abstract RNF20, an E3 ligase critical for monoubiquitination of histone H2B at lysine 120 (H2Bub), has been implicated in the regulation various cellar processes; however, its physiological roles adipocytes remain poorly characterized. Here, we report that adipocyte-specific knockout Rnf20 (ASKO) mice led to progressive fat loss, organomegaly and hyperinsulinemia. Despite signs hyperinsulinemia, normal insulin sensitivity improved glucose tolerance were observed young aged CD-fed ASKO mice. In addition, high-fat diet-fed developed severe liver steatosis. Moreover, extremely sensitive a cold environment due decreased expression levels brown adipose tissue (BAT) selective genes, including uncoupling protein 1 ( Ucp1 ), impaired mitochondrial functions. Significantly peroxisome proliferator-activated receptor gamma Pparγ ) gonadal white tissues (gWAT) from mice, suggesting regulates adipogenesis, least part, through . Rosiglitazone-treated exhibited increased mass compared non-treated Collectively, our results illustrate role RNF20 control development function.