Overexpression of CPE-ΔN predicts poor prognosis in colorectal cancer patients

• DOI: 10.1007/s13277-013-0952-3
• Publication Date: 2013-07-13T01:38:52Z
• Authors: Kun Zhou; Hongyan Liang; Yang Liu; Chun Yang; Peijia Liu; Xiaofeng Jiang
• Keywords: Male; 0303 health sciences; Reverse Transcriptase Polymerase Chain Reaction; Blotting, Western; Carboxypeptidase H; Kaplan-Meier Estimate; Middle Aged; Prognosis; Gene Expression Regulation, Enzymologic; Tumor Burden; 3. Good health; Gene Expression Regulation, Neoplastic; Isoenzymes; Alternative Splicing; 03 medical and health sciences; Lymphatic Metastasis; Multivariate Analysis; Biomarkers, Tumor; Humans; Female; Neoplasm Recurrence, Local; Colorectal Neoplasms; Proportional Hazards Models

Carboxypeptidase E (CPE) is one of the most important carboxypeptidases involved in biosynthesis numerous peptide hormones and neurotransmitters has an role endocrine regulation. A splice variant CPE (CPE-ΔN) been detected mechanism CPE-ΔN action tumorigenesis studied many different cancers. The aim this study was to examine expression human colorectal cancer (CRC) evaluate its possible use as a potential prognostic marker. Two hundred nineteen primary tumors corresponding normal tissues were included study. We have analyzed isoform by qRT-PCR Western blot 219 CRC patients. Correlations between mRNA clinicopathological variables determined with chi-square tests. Survival probabilities using Kaplan-Meier analysis, univariate multivariate analyses factors performed Cox regression model. Our results show that overexpressed tumor tissue high closely correlated differentiation, pT classification, pN recurrence, lymph node metastasis (P = 0.042, 0.036, 0.031, 0.006, 0.008, respectively). However, no correlation observed age, gender, localization, gross features, size. In addition, patients had significantly shorter survival < 0.001, logrank test). Tumor feature, metastasis, status associated poor prognosis after performing analysis. High also identified independent factor analysis 0.011). Based on these results, we can conclude might be marker for