The effects of STAT3 and Survivin silencing on the growth of human bladder carcinoma cells

• DOI: 10.1007/s13277-014-1704-8
• Publication Date: 2014-02-11T01:48:27Z
• Authors: Baogang Zhang; Zhihua Lu; Yuchuan Hou; Jinghai Hu; Chunxi Wang
• Keywords: Survivin; Human bladder

Despite accumulating evidence suggesting a critical role of signal transducer and activator transcription 3 (STAT3) Survivin in human bladder cancer, the effects silencing these genes on proliferation T24 carcinoma cells remain unknown. Here, we investigated inhibitory STAT3 or vitro vivo growth cells. Small interfering RNA (siRNA) vectors targeting STAT3, Survivin, both were designed synthesized. The recombinant plasmid DNA constructs confirmed by sequencing. They then transiently transfected into cells, mRNA protein expressions determined using real-time quantitative reverse polymerase chain reaction (qRT-PCR) Western blot, respectively. Cell was evaluated 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. growth-inhibition siRNA assessed cancer mouse model. tumor weight size recorded 4 weeks post-inoculation. We successfully synthesized four Survivin. STAT3-3 Survivin-4 most efficient reducing expression, constructed vector containing two sequences together (STAT3-Survivin siRNA) found that single efficiently silenced expression. Moreover, significantly suppressed compared to controls (P<0.05). Our findings indicated downregulation can suppress no additive observed when knocked down together, they work same signaling pathway These results provide valuable insights understanding pathways involved during tumorigenesis cancer.