Re-sensitization of 5-FU resistance by SPARC through negative regulation of glucose metabolism in hepatocellular carcinoma

• DOI: 10.1007/s13277-014-2633-2
• Publication Date: 2014-09-24T18:55:55Z
• Authors: Hong-Wei Hua; Feng Jiang; Qian Huang; Zhi-Jun Liao; Gang Ding
• Keywords: Carbohydrate Metabolism; Osteonectin; Matricellular protein

Secreted protein, acidic and rich in cysteine (SPARC), a calcium-binding matricellular glycoprotein, is implicated the progression of many cancers. Currently, there growing evidence for important functions SPARC variety cancers its role cancer depends on tumor types. In this study, we reported negatively regulated glucose metabolism hepatocellular carcinoma (HCC). Overexpression inhibited uptake lactate product through downregulation key enzymes metabolism. On other hand, knock down reversed phenotypes. Meanwhile, exogenous expression HepG2 cells resulted tolerance to low was correlated with AMPK pathway. Interestingly, 5-fluorouracil (5-FU)-resistant showed increased downregulated levels. Finally, overexpression re-sensitize 5-FU-resistant 5-FU inhibition glycolysis both vitro vivo. Our study proposed novel function regulation will facilitate development therapeutic strategies treatments liver patients.