MiR-32 induces cell proliferation, migration, and invasion in hepatocellular carcinoma by targeting PTEN

• DOI: 10.1007/s13277-015-3124-9
• Publication Date: 2015-02-02T20:19:55Z
• Authors: Shi-yan Yan; Mei-mei Chen; Guang-ming Li; Yu-qin Wang; Jian-gao Fan
• Keywords: 0301 basic medicine; Carcinoma, Hepatocellular; Liver Neoplasms; PTEN Phosphohydrolase; 3. Good health; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Humans; Neoplasm Invasiveness; 3' Untranslated Regions; Cell Proliferation; Signal Transduction

MicroRNAs (miRNAs) regulate gene expression by inhibiting translation of target messenger RNAs (mRNAs) through pairing with miRNA recognition elements (MREs), usually in 3'-UTRs. miRNAs are involved the pathogenesis several types cancers. Specifically, microRNA-32 (miR-32) is overexpressed colorectal carcinoma, wherein accumulating evidence indicates that it functions as an oncogene. However, function miR-32 hepatocellular carcinoma (HCC) has not been totally elucidated. In present study, we found was up-regulated HCC tissue and cell lines, inversely phosphatase tensin homolog (PTEN) decreased. Besides, miRNA-32 down-regulates PTEN binding to 3'-UTR mRNA from luciferase reporter assay, level could affect proliferation, migration, invasion liver cancer lines via PTEN/Akt signaling pathway. Down-expression significantly attenuate inhibitory effects knockdown on cells, suggesting be a potential for treatment.