RNA interference against TMEM97 inhibits cell proliferation, migration, and invasion in glioma cells

• DOI: 10.1007/s13277-015-3552-6
• Publication Date: 2015-05-22T10:29:12Z
• Authors: Guanzhong Qiu; Wei Sun; Yongxiang Zou; Zheng Cai; Peng Wang; Xianbin Lin; Jinxiang Huang; Lei Jiang; Xuehua Ding; Guohan Hu
• Keywords: U87

Gliomas are the most common form of primary brain tumor in adult central nervous system. Altered expression and prognostic value transmembrane protein 97 (TMEM97) has been recently reported different types human tumors. However, association TMEM97and glioma is poorly defined. Here, we that TMEM97 was significantly increased tissues compared to non-tumorous tissues. Furthermore, levels were progressively with increasing histologic grade glioma. Higher level correlated shorter survival time patients Downregulation through RNA interference inhibited cell proliferation G1/S transition two lines, U87 U373. More importantly, silencing induced a significant decrease key regulators, cyclin D1, E, CDK2, CDK4. Additionally, downregulation cells notably repressed migration invasion. Further analysis suggested decreased invasion associated alterations EMT markers, including E-cadherin, β-catenin, Twist. Since seems be oncogenic potential glioma, suppression its can inhibit cancer growth metastasis, may therapeutic target