Down-regulation of Sphk2 suppresses bladder cancer progression

• DOI: 10.1007/s13277-015-3818-z
• Publication Date: 2015-07-29T22:14:41Z
• Authors: Erlin Sun; Wenbo Zhang; Lining Wang; Aixiang Wang; Chengquan Ma; Mingde Lei; Xiaodong Zhou; Yan Sun; Bingxin Lu; Liwei Liu; Ruifa Han
• Keywords: 0301 basic medicine; Down-Regulation; Apoptosis; Up-Regulation; 3. Good health; Gene Expression Regulation, Neoplastic; Inhibitory Concentration 50; Phosphotransferases (Alcohol Group Acceptor); 03 medical and health sciences; Urinary Bladder Neoplasms; Cell Movement; Cell Line, Tumor; Disease Progression; Humans; Neoplasm Metastasis; RNA, Small Interfering; Cell Proliferation

Bladder cancer is the second most common urological malignancy around world and by far frequent in China. The abnormal expression of sphingosine kinase 2 (SphK2) associated with tumor progression a poor patient survival rate, however, effect SphK2 on bladder cells remains unclear. aim paper was to study role cell proliferation, metastasis, apoptosis vitro. Our results showed that up-regulated tissues compared corresponding adjacent non-neoplastic tissues, level significantly higher human comparison normal epithelial cells. Silencing could inhibit proliferation ability T24 In addition, knockdown induce significant increase number apoptotic Furthermore, transwell assay also migration inhibition siRNA transfectant lines transfected NC. Thus, this suggested may provide promising treatment for patients.