A new application of plant virus nanoparticles as drug delivery in breast cancer

• DOI: 10.1007/s13277-015-3867-3
• Publication Date: 2015-08-18T05:15:30Z
• Authors: Neda Esfandiari; Mohsen Karimi Arz...; Masoud Soleimani; Mina Kohi-Habibi; Winnie E. Svendsen
• Keywords: Potato virus X; Conjugate

Nanoparticles based on non-pathogenic viruses have opened up a novel sector in nanotechnology. Viral nanoparticles plant clear advantages over any synthetic as they are biocompatible and biodegradable self-assembled can be produced inexpensively large scale. From several such under-development platforms, only few been characterized the target-specific drugs into cells. Potato virus X is presented carrier of chemotherapeutic drug Herceptin that currently used targeted therapy (HER2+) breast cancer patients. Here, we formed from potato to conjugate (Trastuzumab) monoclonal antibody new option specific targeting cancer. Bioconjugation was performed by EDC/sulfo-n-hydroxysuccinimide (sulfo-NHS) two-step protocol. Then, efficiency conjugation investigated different methods, including sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, ELISA, Zetasizer, transmission electron microscopy. SDS-PAGE blot analysis confirmed an 82-kDa protein band resulted (PVX) coat (27 kDa) heavy chain (55 kDa). Zeta potential values for conjugated particles, PVX, HER were −7.05, −21.4, −1.48, respectively. We PVX-Herceptin induce SK-OV-3 SK-BR-3 cells (HER2 positive cell lines) apoptosis. therefore counted measured apoptosis flow cytometry assay, then compared with alone. Based our data, PVX Herceptin. This study suggests conjugates enable become more therapeutic tools.