Inhibition of pentose phosphate pathway suppresses acute myelogenous leukemia

• DOI: 10.1007/s13277-015-4428-5
• Publication Date: 2015-11-23T22:46:05Z
• Authors: Yan Chen; Qian Xu; Dexiang Ji; Yanlin Wei; Huamei Chen; Tingting Li; Bolin Wan; Liya Yuan; Ruibin Huang; Guoan Chen
• Keywords: 0301 basic medicine; Gene Expression Regulation, Leukemic; Gene Expression Profiling; Computational Biology; Genetic Variation; Prognosis; 3. Good health; Pentose Phosphate Pathway; Leukemia, Myeloid, Acute; 03 medical and health sciences; 6-Aminonicotinamide; Glucose; Cell Movement; Cell Line, Tumor; Humans; Oxidation-Reduction; Cell Proliferation

Pentose phosphate pathway (PPP) is a metabolic that generates NADPH and pentose. PPP genes have been reported to be primarily or secondarily upregulated in many cancers. We aimed study the general alteration of acute myelogenous leukemia (AML). performed data mining analysis Cancer Genome Atlas (TCGA) AML dataset for genetic gene set. In vitro studies including proliferation, migration, invasion assays, together with metabolite consumption oxidation were performed. 61 % patients AML. The majority altered cases expression changes measured by RNA sequencing. Expressions critical such as G6PD, PFKL, PFKP, PGLS consistently all cases. Altered not associated survival disease relapse. inhibition using 6-aminonicotinamide (6AN) increases glucose oxidative metabolism 6AN decreased increased fatty acid oxidation. Here, we showed suppressed growth, AML, but colony formation. plays an important role Our results could contribute development novel targeted treatment.